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Home > chinese-english > "pokeweed antiviral protein" in English

English translation for "pokeweed antiviral protein"

美洲商陆抗簿蛋白

Related Translations:
pokeweed:  n.【植物;植物学】美国商陆 (Phytolacca americana) 〔产于北美,其根和果有毒〕。
virginian pokeweed:  美国商陆
pokeweed pokeroot:  美洲商陆
pokeweed mitogen:  美洲商陆有丝分裂原商陆促分裂原商陆丝裂素
pokeweed family:  商陆科
pokeweed mosaic virus:  商陆花叶病毒商陆花叶簿
pokeweed mosaic potyvirus:  商陆花叶马铃薯y病毒商陆花叶马铃薯y簿
pokeweed crinkle leaf virus:  商陆皱叶病毒商陆皱叶簿
Example Sentences:
1.Someone tried this way but the yield is low . francis expressed truncated mutant pokeweed antiviral protein gene in pichia pastoris
目前已经有人在这方面做出了尝试,但蛋白表达产量还不高。
2.Many studies revealed that pokeweed antiviral protein ( pap ) can control plant virus effectively
许多研究发现美洲商陆抗病毒蛋白( pokeweedantiviralprotein , pap )对植物病毒具有良好的控制效果。
3.To find out the difference between mut + and muts recombinants we compared their expression of pokeweed antiviral protein in the same conditions
在相同的培养条件下比较了mut ~ +和mut ~ s重组菌株表达pap的异同。
4.Linearized the expression vector ppic9k - p including the truncated mutant pokeweed antiviral protein ( pap ) gene by restriction endonuclease sal i and transformed it electrically into pichia pastoris gs115 . mut + recombinants were selected by pcr and high yield mut + recombinant was picked out by double film immunoblotting
本研究将含有n末端信号肽和c末端毒性区缺失的pap基因的表达载体ppic9k - p用sali酶切线性化后,通过电击转化整合p . pastorisgs115菌株细胞中。
5.The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector . the sequencing results showed that pap gene had 99 . 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ) . the iptg - inducible expression vector containing the pap gene was constructed and transferred into e . coli bl21 ( de3 ) - plyss
将缺失型pap基因克隆到植物表达载体pbi121中,通过液氮冷冻法将重组质粒转入农杆菌lba4404细胞中,然后采用叶盘法,在该农杆菌的介导下将pap基因导入普通烟草中,经过卡那霉素抗性筛选,最后获得了转pap基因的工程烟草植株,摩擦接种烟草花叶病毒( tmv ) ,与非转基因烟草相比,能够推迟症状表现达2月之久,说明pap基因能够在其它植物体内产生有活性的高抗病毒的蛋白质。
6.Sds - page results showed that as to mut + recombinant highest yield was obtained after 4 days inducing and with the culture time prolonged it reduced . pokeweed antiviral protein gene expressed well when methanol concentration reached 10g / l . pokeweed antiviral protein obtained high yield in thin acidic culture medium ( ph6 . 0 - 6 . 4 ) and its quantity in total mass of secrete protein exceeded 30 %
Sds - page分析结果表明, mut ~ +组菌株在甲醇诱导第四天后pap在培养液中积累量达到最高水平,延长培养时间会导致产量下降;在10g / l的甲醇浓度诱导下, pap的表达量达到最高;培养基ph值在偏酸性条件下( 6 . 0 - 6 . 4 ) pap的表达量都维持在较高的水平。
Similar Words:
"pokeroot" English translation, "pokerpicture" English translation, "pokesl" English translation, "pokethrough" English translation, "pokeweed" English translation, "pokeweed crinkle leaf virus" English translation, "pokeweed family" English translation, "pokeweed mitogen" English translation, "pokeweed mosaic potyvirus" English translation, "pokeweed mosaic virus" English translation